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Table 2 Targeted Q-PCR analysis of mammary glands after 3, 4, or 10 weeks and mammary tumors on HFD versus LFD

From: Pubertal high fat diet: effects on mammary cancer development

Growth factor genes

Fold change HFD versus LFD

Symbol

Description

3 wks MG

4 wks MG

10 wks MG

Tumor

Fgf18

Fibroblast growth factor 18

2.0

-

-

-

Il4

Interleukin 4

2.0

-

-

-

Bdnf

Brain derived neurotrophic factor

-

2.4

-

-

Lefty2

Left-right determination factor 2

-

-2.5

-

-

Lif

Leukemia inhibitory factor

-

2.8

-

-

RANKL

Receptor activator of nuclear factor kappa-B ligand

-

17

-

-

Bmp2

Bone morphogenetic protein 2

-

-

2.0

-

Bmp3

Bone morphogenetic protein 3

-

-

2.4

-

Fgf10

Fibroblast growth factor 10

-

-

2.0

-

Gdf10

Growth differentiation factor 10

-

-

1.9

-

Gdf5

Growth differentiation factor 5

-

-

2.2

-

Nodal

Nodal

-

-

2.4

-

Pgf

Placental growth factor

-

-

2.5

-

Tgfa

Transforming growth factor alpha

-

-

1.9

-

Tgfb1

Transforming growth factor, beta 1

-

-

1.9

-

Vegfa

Vascular endothelial growth factor A

-

-

2.1

-

Il1a

Interleukin 1 alpha

-

-

3.0

-

Il1b

Interleukin 1 beta

-

-

2.1

-

Il2

Interleukin 2

-

-

3.0

-

Il7

Interleukin 7

-

-

3.4

-

Cxcl12

Chemokine (C-X-C motif) ligand 12

-

-

1.8

-

Bmp7

Bone morphogenetic protein 7

-

-

-

3.2

Inha

Inhibin alpha

-

-

-

2.2

Ntf3

Neurotrophin 3

-

-

-

53.4

Bmp10

Bone morphogenetic protein 10

-

-

-

-3.4

  1. N = 3 high fat diet (HFD) mice, n = 3 low fat diet (LFD) mice, n = 3 HFD mice with early developing tumors (HFD-E), n = 3 LFD mice with tumors. P ≤0.05 for all genes listed.