Figure 1

Characteristics of tumor development in high fat diet- versus low fat diet-fed mice. (A) Kaplan-Meier plot of all tumors developing in high fat diet (HFD)- and low fat diet (LFD)-fed mice. Time = number of days post last 7,12-dimethylbenz[a]anthracene (DMBA) treatment (HFD mice, n = 95; LFD mice, n = 87) (B) Time-course of epithelial proliferative lesion development. More hyperplastic and precancerous lesions developed in HFD-fed DMBA-treated mice at 8 and 14 weeks post first DMBA treatment. Bars represent mean ± standard error of the mean of lesions per mouse; n = 5 mice at each time for HFD and LFD. ^*P = 0.05; ^**P = 0.003. (C) Epithelial proliferative lesions comprised (i) terminal duct hyperplasia, (ii) lobular hyperplasia, and (iii) mixed dysplasia. Scale bar = 1 mm.