Figure 1

HDL ₃ induces migration and acts as a signaling molecule in MDA-MB-231 and MCF7 cells. Migration of MCF7 (A) and MDA-MB-231 (B) cells was induced by HDL₃. Migration of MCF7 and MDA-MB-231 cells was increased when HDL₃, but not LDL, was used as a chemoattractant compared with CTL (1% BSA alone) in Boyden Chamber assays. Stained cells were quantified by using phase-contrast microscopy. Columns represent the mean number of migrated cells; bars represent ± standard deviation (SD) (n = 3). The results obtained with CTL and HDL₃ groups are significantly different. No statistical difference was found between CTL and LDL groups. (A) *P < 0.05, Student's t test. (B) **P < 0.01, versus 1% BSA by Student's t test). Results shown are representative of three independent experiments. (C). MDA-MB-231 and MCF7 cells were incubated for the indicated amounts of time with 100 μg/ml HDL_3. Whole-cell lysates were analyzed by Western blotting for the indicated proteins. The arrow indicates the protein corresponding to phospho-Akt. Results are representative of three independent experiments. (D) MDA-MB-231 and MCF7 cells were incubated for the indicated amounts of time with 100 μg/ml LDL. Whole-cell lysates were analyzed by Western blotting for the indicated proteins. The arrow indicates the protein corresponding to phospho-Akt. Results are representative of three independent experiments.