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Figure 2 | Breast Cancer Research

Figure 2

From: Parthenolide and costunolide reduce microtentacles and tumor cell attachment by selectively targeting detyrosinated tubulin independent from NF-κB inhibition

Figure 2

Tubulin detyrosination is independent of NF-κB activation. (A) NF-κB -luciferase reporter adenovirus infected MDA-MB-157 and Bt-549 show that a four-hour treatment of parthenolide (Parth; 10 μM, 25 μM) and costunolide (Cost; 10 μM, 25 μM) inhibit TNF-α-induced (100 ng/ml) NF-κB activation at concentrations that reduced detyrosinated tubulin. Resveratrol (ResV; 50 μg/ml, 100 μg/ml) inhibits NF-κB but does not affect detyrosination. Colchicine (Col; 50 μM) and Taxol (Tax; 0.5 μg/ml) do not inhibit NF-κB at concentrations that affect detyrosinated tubulin. (B) Cell viability assay shows that non-toxic drug concentrations are used. All compounds are expressed as a % of vehicle (set at 100%; horizontal dotted line). NF-κB, nuclear factor-kappaB.

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