MCF10A series cocultures present differentially secreted cytokines and MET receptor status. (A) A variety of cytokines are overexpressed in the direct cocultures of all three cell lines. The number of cytokines overexpressed increases with progression. The cytokine most strongly overexpressed was hepatocyte growth factor (HGF). (B) MET RNA fold-change expression relative to the corresponding cell line in monoculture over a 48-hour period. We observe a sharp increase of transcript in the MCF10DCIS cells when in coculture after 6 hours. (C) MET protein fold-change expression relative to the corresponding cell line in monoculture over a 48-hour period. The protein increase is delayed 6 hours when compared with RNA expression due to the delay in translation. FGF, fibroblast growth factor; GCP, granulocyte chemotactic peptide; GCSF, granulocyte colony-stimulating factor; MCP, monocyte chemotactic peptide; MIG, monokine induced by interferon-gamma; RANTES, regulated on activation, normal T-cell expressed and secreted; RMF, reduction mammoplasty fibroblast.