Figure 1

Tumor onset, multiplicity and cell proliferation were suppressed inCOX-2^MECKO tumors. COX-2^MECKO tumors aredenoted as KO. (A) Percent of tumor free mice against weeks of age. Meantumor free time for COX-2^MECKO mice was 29 weeks versus 23 weeks forWT (left graph, n = 13 to 19). The right graph shows tumormultiplicity as number of tumors per mice at necroscopy (n = 14 to18). (B) Gene expression levels of Ki67 (proliferation), Caspase3(apoptosis) and Lc3 (autophagy) in whole tumors by Q-PCR (n = 8 to18). (C) Immunohistochemistry staining for Ki67 (dark red-brown) insections of paraffin embedded WT and COX-2^MECKO tumors (image shownis representative of n = 4). Cell nuclei are counterstained withhematoxylin. The bar on the KO panel indicates 20× magnification. Data incolumn graphs are mean ± sem. P values are compared to WT; *P < 0.05. COX, cyclooxygenase; KO, knock out; WT, wild type.