Figure 5

miR-510 regulates endogenous PRDX1 protein expression. (A) Western blot and (B) quantitative PCR analysis of endogenous peroxiredoxin 1 (PRDX1) expression in MCF10A, MCF7 and MDA MB 231 cells stably infected with scrambled control (scr) or miR-510 (510). (A) GAPDH is shown as a loading control. (B) Relative expression is normalized to GAPDH; scr - dark gray bars, miR-510 - light gray bars. (C) Western blot and (D) quantitative PCR analysis of CAMA-1 and BT549 cells transiently transfected with increasing concentrations of antisense oligonucleotides to miR-510(ASO-510) or antimiR-510 vector (anti-510). (C) ACTIN is shown for as a loading control. (D) Relative expression is normalized to GAPDH; scr - dark gray bars, ASO-510 (150 nM) anti miR-510 (1 ug) - light gray bars, ASO-510 (450 nM) anti miR-510 (2 ug)- black bars). Fold change in PRDX1 protein levels compared to scr control were quantified and are shown under each Western blot image.