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Figure 8 | Breast Cancer Research

Figure 8

From: LOXL2 induces aberrant acinar morphogenesis via ErbB2 signaling

Figure 8

LOXL2 expression induces ErbB2 activation through production of 2 O 2 . (A)Production of H2O2 on Matrigel was measured as an activity read-out in the 10A cont and 10A L2 cells, by using a commercially available kit and following the manufacturer's instructions (Abcam). 10A L2 cells produced higher levels of H2O2, as expected, because of LOXL2 overexpression. (B) The 200 U/ml catalase (+cat) was added to remove H2O2 produced by LOXL2. Western blotting revealed that pErbB2 was decreased in 10A L2 cells treated with catalase (L2+cat) in comparison with untreated cells (L2). (C) Addition of H2O2 to 10A cont cells strongly induced ErbB2 activation. Taken together, these results indicate that, by removing H2O2, we can significantly abrogate ErbB2 activation in 10A L2 cells, and addition of H2O2 activated ErbB2.

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