Figure 6

PKD1-dependent effects of decitabine treatment on metastasis progression. (A) The presence of metastasis was detected using the IVIS Spectrum Imaging System in vivo at day 77. Representative pictures from each treatment group are shown. PKD1, protein kinase D1; scr-shRNA, scrambled control short hairpin RNA. (B) The presence of pulmonary metastasis was detected by immunostaining with an antibody specific for human vimentin (detects human cancer cells). (C) The number of pulmonary metastases per square millimeter was quantified from five fields in each lung. (D) The area of metastatic nodules in each section was quantified from five fields in each lung. For (C) and (D), the values are means ± SEM. P values were calculated using a two-tailed Student’s t-test and standard deviation. *P ≤ 0.005 indicates statistical significance.