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Figure 5 | Breast Cancer Research

Figure 5

From: Pharmacologic reversion of epigenetic silencing of the PRKD1promoter blocks breast tumor cell invasion and metastasis

Figure 5

PKD1-dependent effects of decitabine treatment on local invasion. (A) After control (saline) or decitabine treatment, primary tumors obtained with the MDA-MB-231 scrambled control short hairpin RNA (scr-shRNA) or protein kinase D1 (PKD1) shRNA 1 or 2 cell line and were either stained with hematoxylin and eosin (H&E) or analyzed for matrix metalloproteinase 9 (MMP9) expression (anti-MMP9). Representative pictures are shown. (B) The number of MMP9-positive cells per square millimeter was determined using the Aperio Positive Pixel Count algorithm in ImageScope viewing software. P values were calculated using Student’s t-test in GraphPad Prism version 5 software. *P ≤ 0.005 indicates statistical significance.

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Breast Cancer Research

ISSN: 1465-542X

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