Evidence for a multipotent mammary progenitor with pregnancy-specific activity

Breast Cancer Research

Table 1 Limiting dilution transplants of MMTVrtTA/H2BGFP populations

Population	Number of cells	Number of glands	MRU frequency (confidence interval)
Virgin recipients
a. H2GFP ⁺ /CD24 ⁺ /CD29 ⁺	10	3/16	1/105 (70 - 160)
	20	5/12
	50	3/12
	100	6/10
	200	9/12
b. H2GFP ^- /CD24 ⁺ /CD29 ⁺	10	2/12	1/80 (60 - 130)
	20	2/14
	50	5/12
	100	9/12
	200	9/10
c. H2GFP ⁺ /CD24 ⁺ /CD29 ^lo	10	0/14	1/1,600 (500 - 4,900)
	20	0/12
	50	0/12
	100	1/12
	200	2/14
d. H2GFP ^- /CD24 ⁺ /CD29 ^lo	10	0/14	None
	20	0/12
	50	0/12
	100	0/14
	200	0/14
+ Pregnancy
e. H2GFP ⁺ /CD24 ⁺ /CD29 ⁺	10	1/4	1/60 (35 - 100)
	20	3/6
	50	5/8
	100	5/8
	200	6/6
f. H2GFP ^- /CD24 ⁺ /CD29 ⁺	10	3/8	1/140 (80 - 240)
	20	3/6
	50	2/8
	100	4/8
	200	4/8
g. H2GFP ⁺ /CD24 ⁺ /CD29 ^lo	10	1/6	1/350 (160 -790)
	20	0/8
	50	1/6
	100	1/8
	200	3/6
h. H2GFP ^- /CD24 ⁺ /CD29 ^lo	10	0/6	None
	20	0/8
	50	0/6
	100	0/6
	200	0/6
Virgin Recipients vs. Pregnancy - 2 ^nd Experimental Set
i. H2GFP ⁺ /CD24 ⁺ /CD29 ^lo	10	0/14	1/3,800 (500 - 27,000)
Virgin	20	0/12
	50	1/12
	100	0/12
	200	0/8
j. H2GFP ⁺ /CD24 ⁺ /CD29 ^lo	10	2/12	1/280 (170 - 480)
+Pregnancy	20	2/14
	50	4/14
	100	4/14
	200	4/14

Mammary repopulating ability of H2BGFP⁺/CD24⁺/CD29⁺ (a, e), H2BGFP^-/CD24⁺/CD29⁺ (b, f), H2BGFP⁺/CD24⁺/CD29^lo (c, g, i) and H2BGFP^-/CD24⁺/CD29^lo (d, h, j) populations in virgin mice (a-d, i) and pregnant mice (e-h, j). H2BGFP, histone 2B-eGFP; MMTV, mouse mammary tumor virus promoter; MRU, mammary reproducing unit; rtTA, reverse tetracycline transactivator.

ISSN: 1465-542X