Figure 4
From: Evidence for a multipotent mammary progenitor with pregnancy-specific activity
In vivo outgrowth of H2BGFP+/CD24+/CD29 locells. Outgrowths from H2BGFP+/CD24+/CD29lo cells were stained with Carmine Alum for whole-mount analysis, then sectioned and immunostained to analyze their epithelial origin and composition. (a) mammary fat pad whole mount (b) DAPI, GFP and β1 integrin staining (c) CK8, GFP and p63 staining (d) CK14, GFP and β1 integrin staining (e) DAPI, GFP and milk staining. Panel b, 10× magnification. Panels c-e, 60× magnification. Arrowheads indicate representative co-expressing cells. DAPI, 4',6-diamidino-2-phenylindole; GFP, green fluorescent protein; H2BGFP, histone 2B-eGFP.