Figure 8
HIF-1α and GPER are recruited to the VEGF promoter sequence. CAFs treated for 1 h with 100 μM CoCl2 were submitted to the chromatin immunoprecipitation procedure using anti-HIF-1α (a) or anti-GPER (b) antibodies. For knockdown experiments, CAFs were transfected with control shRNA and shGPER (c) or with control shRNA and shHIF-1α (d), treated for 1 h with 100 μM CoCl2 and then submitted to the chromatin immunoprecipitation procedure using anti-HIF-1α (c) or anti-GPER (d) antibodies. The amplified sequences were evaluated by real-time PCR. Each data point represents the mean ± SD of three independent experiments. (○) P < 0.05 for cells receiving vehicle vs CoCl2 treatment.