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Figure 6 | Breast Cancer Research

Figure 6

From: Autocrine IGF-I/insulin receptor axis compensates for inhibition of AKT in ER-positive breast cancer cells with resistance to estrogen deprivation

Figure 6

IGFBP-3 blocks AZD5363-induced phosphorylation of IGF-IR/InsR and AKT. A) MCF-7/LTED cells were treated overnight with serum-free medium ± 1.5 µg/ml IGFBP-3, followed by treatment for 15 minutes ± 100 ng/ml IGF-I or IGF-II. Protein lysates were analyzed by immunoblot using the indicated antibodies. B) MCF-7/LTED cells in 10% DCC-FBS were treated ± 1.5 µg/ml recombinant IGFBP-3 for 1 hour followed by 2 µM AZD5363 for 24 hours. Cell lysates (0.5 mg) were prepared and analyzed by phospho-RTK arrays. C) MCF-7/LTED cells in 10% DCC-FBS were treated ± 1.5 µg/ml IGFBP-3 for 1 hour followed by 2 µM AZD5363 for 24 hours. Protein lysates were prepared and analyzed by immunoblot using the indicated antibodies. DCC-FBS, dextran/charcoal-treated fetal bovine serum; IGF, insulin-like growth factor; LTED, long-term estrogen deprivation.

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