Figure 4

Cyclin D1 is a downstream mediator in TGFβ-regulated actin reorganization and invadopodia formation. (A) Scr siRNA- or cyclin D1 siRNA-transfected SCP2 cells were treated with or without 5 ng/ml transforming growth factor beta (TGFβ) for 24 hours. Immunocytochemistry was performed using F-actin (green) antibody, vimentin (red) antibody and DAPI (blue). Co-localizaton (yellow) between F-actin and vimentin is represented by overlay. The scale bar is 10 µm. (B) Scr siRNA- or cyclin D1 siRNA-transfected SCP2 cells were cultured on the top of growth factor-reduced Matrigel and then treated with or without 5 ng/ml TGFβ for 24 hours. Immunocytochemistry was performed using F-actin (green) and cyclin D1 (red) antibodies and DAPI (blue).