Figure 7
From: Amphiregulin mediates progesterone-induced mammary ductal development during puberty
17-β-Estradiol regulates amphiregulin, and progesterone regulates both amphiregulin and RANKL in the prepubertal mammary gland. Prepubertal 3-week-old BALB/c mice were OVX, allowed to recover for 2 weeks, and treated once with vehicle control (C), E2, or P, as described in the Materials and Methods section. (A) Expression analysis of amphiregulin (AREG) by RT-PCR. Fold change is relative to control treatment. (B) Dual immunofluorescence detection of AREG (green) and either ERα (red, on left) or PR (red, on right). AREG expression increased in response to E2 or P in ERα-expressing cells and was most strongly expressed in end buds in response to P treatment. Nuclei were counterstained with DAPI (blue). Scale bar, 25 μm.