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Figure 6 | Breast Cancer Research

Figure 6

From: Amphiregulin mediates progesterone-induced mammary ductal development during puberty

Figure 6

Both 17-β-estradiol and progesterone-induced responses in the prepubertal mammary gland occur through their cognate receptors. (A) Representative whole mounts from prepubertal 3-week-old BALB/c mice that were OVX, allowed to recover for 2 weeks, and then treated once with vehicle control (C) + ICI 182,780 (ICI), E2 + ICI, or P + ICI, as described in the Materials and Methods section. Scale bar, 1 mm. Inset shows higher magnification of end buds (EBs), which were present in only the P + ICI treatment group. Scale bar, 1 mm. (B) Effect of RU486 implant on P-induced EB formation. RU486 pellet implanted in the right mammary gland number 4, and control pellet implanted in the contralateral number 4 mammary gland. Implants are outlined with dashed lines and noted with an asterisk. Note stimulated EB in control L4 gland and nonstimulated duct ends (arrows). Scale bar, 2 mm. (C) Proliferation analysis by dual immunofluorescence detection of BrdU and PR. Total percentage BrdU-positive cells and percentage BrdU-positive cells coexpressing PR in ducts and end buds after C, E2, P, or E2+P treatment are presented. The percentage of BrdU-positive cells in ducts increased in response to E2, P, or E2+P treatment compared with control (*P < 0.05). The percentage of BrdU-positive cells increased in end buds compared with ducts in E2, P, or E2+P (#P < 0.05).

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