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Figure 3 | Breast Cancer Research

Figure 3

From: Amphiregulin mediates progesterone-induced mammary ductal development during puberty

Figure 3

Iressa inhibits both 17-β-estradiol and progesterone-induced proliferation and EB formation. Pubertal 4-week-old BALB/c mice were OVX, allowed to recover for 3 weeks, and then treated for 5 days with vehicle control (C), E2 +/- Iressa (300 mg/kg), or P +/- Iressa, as described in the Materials and Methods section. (A) EB formation after 5 days E2, E2+Iressa, P, or P+Iressa. Note the absence of EBs in Iressa-treated mammary glands. Scale bar, 2 mm. (B) End bud quantitation. Values represent the mean ± SEM number of end buds in a number 4 mammary gland (n = 5 mice). (C) The percentage of proliferating cells in ducts and EBs was determined by immunofluorescence detection of BrdU incorporation. P+Iressa treatment reduced proliferation in ducts relative to P treatment (*P < 0.05).

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