Figure 1

Effect of PGE ₂ on HIF-1α expression and nuclear localization in primary human breast ASCs. PGE₂ caused a significant increase in HIF-1α transcript (A) and nuclear protein expression (B). Confocal microscopy demonstrated that HIF-1α (green) is mainly perinuclear in breast ASCs under basal conditions (C, top left) and that PGE₂ stimulates the translocation of HIF-1α to the nucleus (C, top right). Treatment with DMOG (C, bottom left) and DMOG with PGE2 (C, bottom right) caused a much higher HIF-1α staining in the nucleus. The merged lamin B1+B2 nuclear stain (red) and HIF-1α are found as insets at the bottom right of each image. The percentage of cells positive for nuclear HIF-1α was also shown to be significantly increased with PGE₂ treatment (D). vc = vehicle control, n = 3, repeated twice. Confocal images are representative of the majority of cells examined.