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Figure 2 | Breast Cancer Research

Figure 2

From: Vaccinia virus GLV-1h153 is a novel agent for detection and effective local control of positive surgical margins for breast cancer

Figure 2

GLV-1h153 kills and replicated efficiently in breast cancer cell lines in vitro. A. GLV-1h153 kills breast cancer cells in a dose-dependent fashion. Cytotoxicity data of cell lines MDA-MB-231, MDA-MB-468 and HCC38 showed less than 10% viable cells after five days of infection at an MOI of 5. Multiplicity of infection (MOI) = plaque-forming units/cell. Experiment was performed in triplicates. B. GLV-1h153 replicated efficiently in all breast cancer cell lines. All cell lines supported viral replication as assessed by the viral plaque assay. Results demonstrated that in cell line MDA-MB-468, GLV-1h153 reached the highest titer of 2.7 × 107 PFU after 96 hours of infection, representing over a 10,000-fold increase in copy numbers from the initial viral dose. The experiment was performed in triplicates.

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Breast Cancer Research

ISSN: 1465-542X

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