Table 1 List of primers used in this study
PCR | Primer | Sequence (5'-3') | Product size (bp) | PCR cycles | Annealing temperature (°C) |
|---|---|---|---|---|---|
MSP | DACT1m3 | CGGGATAGTAGTAGTCGGC | 118 | 41 | 60 |
DACT1m4 | CGCTAAAACTACGACCGCG | ||||
DACT1u3 | GTTGGGATAGTAGTAGTTGGT | 123 | 41 | 58 | |
DACT1u4 | AAACACTAAAACTACAACCACA | ||||
RT-PCR | DACT1-F | AGGAGAAGTTCTTGGAGGAG | 179 | 32 | 55 |
DACT1-R | TGAGCTAGGCCGACTGTCTG | ||||
GAPDH-F | GGAGTCAACGGATTTGGT | 206 | 23 | 55 | |
GAPDH-R | GTGATGGGATTTCCATTGAT | ||||
Real-time PCR | DACT1-F | GACGAGCAGAGCAATTACACC | 158 | 40 | 60 |
DACT1-R | ACCGTTTGAATGGGCAGA | ||||
β-actin-F | CCTGTGGCATCCACGAAACT | 314 | 40 | 60 | |
β-actin-R | GAAGCATTTGCGGTGGACGAT |