DACT1 was downregulated in primary breast tumors. (A) Analysis of DACT1 expression in normal breast tissues and primary breast tumors by using real-time PCR. (B) Representative immunohistochemical (IHC) staining for DACT1 in paired breast tumors and surgical-margin tissues. Magnification, 400×. (C) The mean optical density of DACT1 expression in each case (left panel). Quantitative analysis of DACT1 expression density is shown as values of mean ± SD (right panel). ***P < 0.001. (D, E) GSA-tumor analysis of DACT1 by using the 1,881-sample breast cancer data set. Box plot of DACT1 expression for tumor samples stratified according to Hu subtypes, ER status, and histologic grade.