Figure 7
From: Endoplasmic reticulum stress induces PRNP prion protein gene expression in breast cancer
ATF6α and XBP1 transactivate PRNP promoter. (A) Ethidium bromide stained gel showing ΔATF6α, sXBP1 and XBP1 amplicons from HEK293 cells co-transfected with the pGL-538 PRNP promoter luciferase reporter and pCGN-ATF6α (1-373) and/or pCGN-sXBP1. (B) Luciferase luminescence generated from HEK293 cells co-transfected as described in A. (C) Ethidium bromide stained gel showing ATF6α and XBP1 amplicons from HEK293 cells co-transfected with the pGL-538 PRNP promoter luciferase reporter and siRNAs against ATF6α or XBP1. (D) Luciferase luminescence generated from HEK293 cells co-transfected as described in C. Statistical analyses on B and D were one-way ANOVA followed by a Tukey-Kramer multiple comparison test.