Figure 1

Oestrogenic effects on cancer stem cells. Cells were cultured for 48 hours in a monolayer in the presence of 1 nM 17β-estradiol or vehicle control and CSC assays were performed in ER positive cells (primary, MCF7, T47D and BT474) and ER negative cells (231). (A) Mammosphere formation, (B) holoclone formation and (C) expression of ESA⁺ CD44⁺ CD24^low were measured. Means plotted ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.