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Table 3 Comparison between CK19 RT-qPCR, multiplex RT-qPCR and AdnaTest for the molecular characterization of CTC in breast cancer.

From: Comparison of three molecular assays for the detection and molecular characterization of circulating tumor cells in breast cancer

  

Multiplex RT-qPCR

Singleplex RT-qPCR

AdnaTest/

CK19

HER-2

MAGE A3

CK19

 
  

Pos.

Neg.

Pos.

Neg.

Pos.

Neg.

Pos.

Neg.

  

Early breast cancer, (number = 254)

GA733-2

Positive

1

8

0

9

0

9

2

7

 

Negative

24

221

28

217

12

233

34

211

 

Concordance (%)

87.4

Pa = 0.897

85.4

Pa = 0.284

91.7

Pa = 0.496

83.9

Pa = 0.481

MUC-1

Positive

0

14

1

13

0

14

1

13

 

Negative

24

215

27

213

12

228

35

205

 

Concordance (%)

84.6

Pa = 0.213

84.3

Pa = 0.633

89.8

Pa = 0.391

81.1

Pa = 0.438

HER-2

Positive

2

24

2

24

0

26

2

24

 

Negative

23

205

26

202

12

216

34

194

 

Concordance (%)

81.5

Pa = 0.698

80.3

Pa = 0.567

85

Pa = 0.231

77.2

Pa = 0.317

  

Verified metastasis, (number = 51)

GA733-2

Positive

14

6

6

14

3

17

15

5

 

Negative

4

27

2

29

1

30

6

25

 

Concordance (%)

80.4

P b < 0.001

68.6

Pb = 0.045

64.7

Pb = 0.287

78.4

P b = 0.000

MUC-1

Positive

14

9

6

17

3

20

14

9

 

Negative

4

24

2

26

1

27

7

21

 

Concordance (%)

74.5

P b = 0.001

62.7

Pb = 0.119

58.8

Pb = 0.316

68.6

P b = 0.012

HER-2

Positive

11

7

5

13

1

17

13

5

 

Negative

7

26

3

30

3

30

8

25

 

Concordance (%)

72.6

P b = 0.006

68.6

Pb = 0.112

60.8

Pb = 1

74.5

P b = 0.001

  1. achi-square test; bFisher's exact test; P < 0.05 is considered as significant.