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Figure 6 | Breast Cancer Research

Figure 6

From: Hormone-sensing cells require Wip1 for paracrine stimulation in normal and premalignant mammary epithelium

Figure 6

Hormone-sensing cells are dependent on Wip1 for their response to Her2/neu activation. (A, B) Confocal immunofluorescence detecting phosphorylated extracellular signal-regulated kinase (P-ERK, red), estrogen receptor-α (ER, green), and cytokeratin-8 (blue) in MMTV (mouse mammary tumor virus)-neu Wip1 wild-type (WT; MMTV-neu, A) or MMTV-neu Wip1-knockout (KO; MMTV-neu, B) mammary epithelium. (C) Quantification of the proportion of ER-positive (ER+) luminal cells positive for phospho-ERK. Values are presented as the mean proportions from three to five mice/group ± SD. **P < 0.001. (D, E) Confocal immunofluorescence detecting phosphorylated signal transducer and activator of transcription 5 (P-STAT5, red), estrogen receptor-α (ER, green), and cytokeratin-8 (CK8, blue) in MMTV-neu Wip1 wild-type (WT; MMTV-neu, D) or MMTV-neu Wip1 KO (KO; MMTV-neu, E) mammary epithelium. Scale bar, 10 μm. Arrows, ER-positive cells; arrowheads, alveolar cells. (F) Quantification of the proportion of ER-positive or ER-negative luminal cells positive for phospho-STAT5. Values are presented as the mean proportions from five mice/group ± SD. ***P < 0.01. (G) Summary of immunostaining intensities for phospho-STAT5 and phospho-ERK in Neu-positive wild-type and Wip1 KO virgin and pregnant mice, and in the context of MMTV-neu. (H) Receptor activator of nuclear factor kappa-B ligand (RANKL) transcription in Neu-positive wild-type (blue bars) and Wip1 KO (green bars) luminal subsets (hormone sensing (HS, red) and alveolar cells (purple)). Data are presented as mean ± SD of two separate sets of wild-type and Wip1 KO animals.

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