Figure 5

Hormone-sensing cells produce less paracrine factors in the absence of Wip1. Quantitative polymerase chain reaction (qPCR) analysis of receptor activator of nuclear factor kappa-B ligand (RANKL; A, B), insulin-like growth factor-2 (IGF-2; C, D), and β-casein (E, F) transcription in wild-type (WT, blue bars) and Wip1-knockout (KO, green bars) luminal subsets (hormone-sensing (HS, red) and alveolar cells (Alv, purple)) obtained from virgin or 7-day pregnant mice. Data are presented as mean ± SD of three to four separate sets of WT and Wip1 KO animals in three separate qPCR experiments. **P < 0.01, and *P < 0.05; n.d., not detectable.