Figure 3
From: Elafin, an inhibitor of elastase, is a prognostic indicator in breast cancer
Elafin and elastase have an inverse pattern of localization. A) Non-tumorigenic breast epithelial cell lines (top panel), ER-positive breast cancer cell lines (middle panel) and ER-negative breast cancer cell lines (bottom panel) were subjected to confocal microscopy to detect the levels of elafin and elastase expression (red) and their localization in the cytoplasm and nucleus (blue). B) The number of cells expressing elafin and elastase for each cell line was quantified. C) Western blots confirm down-regulation of elafin by shRNA constructs, Elafin 1 (lane 3) and Elafin 2 (lane 4), compared to parental and empty vector control cells (lanes 1 and 2, respectively). D) Elastase activity was determined by measuring the amount of substrate cleaved by elastase and was detected by spectrophotometry in 76NE6 and 76NF2V clones with either an empty vector or shRNA against elafin, Elafin 1 and Elafin 2 (*P < 0.05,**P < 0.001).