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Figure 3 | Breast Cancer Research

Figure 3

From: Cell-extrinsic consequences of epithelial stress: activation of protumorigenic tissue phenotypes

Figure 3

COX-2 activity is sufficient to induce phenotypes consistent with desmoplasia. RM9, RM15, RM111, or RM124 human mammary fibroblasts (HMFs) were exposed to exogenous PGE2 or the COX-2 inhibitor, NS398, for 48 hours. mRNA levels for fibronectin (FN1), collagen 1A1 (Col1a1), tenascin C (TenC), and HIF1α were assayed in triplicate, and protein levels for IL-8, IL-6, VEGF, and activin A were analyzed in duplicate for at least three individuals. Relative levels of (A) FN1, Col1a1, TenC, (B) IL-8, IL-6, VEGF, (C) HIF1α, and (D) activin A and COX-2 were expressed relative to controls.

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