Comparative analysis of the human invasion signature (HIS) with the NKI-70-signature. (A) Metastasis-free survival Kaplan-Meier analysis on cases identified as high and low risk by the HIS in the NKI295 cohort (P < 0.0001). The graph is repeated here from Figure 5A for ease of comparison. Also shown is the metastasis-free survival Kaplan-Meier analysis on cases identified as high and low risk by the NKI-70 gene signature in the NKI295 cohort (P < 0.0001). (B) Multivariate Cox proportional hazard regression analysis was performed to evaluate the relation between the HIS and distant metastasis in the NKI295 cohort, incorporating relevant clinical variables as well as the NKI-70 signature (HR, hazard ratio; CI, confidence interval). The NKI-70 signature is a strong predictor, which is expected, because this signature was derived from this same cohort. However, the HIS is significant even in the presence of the NKI-70 signature, indicating that it contains additional prognostic information for this cohort beyond that captured by the NKI-70 signature.