RAD001 in combination with endocrine therapy induces increased pAKT, pERK1/2, and pHER3. Steroid-depleted aromatase-expressing cells were treated for 24 hours with the drug combinations indicated. Whole-cell extracts were assessed for expression of various proteins indicated by using immunoblotting. (A) Effect of RAD001 alone or in combination with the endocrine agents on S6 kinase, ERK1/2, and AKT for the selected cell lines. (B) Effect of RAD001 on IGF1-R signalling. (C) Effect of RAD001 on HER signaling. Figures below each panel, where shown, represent semiquantitative changes in protein expression relative to actin.