Figure 3From: Effectiveness and molecular interactions of the clinically active mTORC1 inhibitor everolimus in combination with tamoxifen or letrozole in vitro and in vivoRAD001 in combination with endocrine therapy induces increased pAKT, pERK1/2, and pHER3. Steroid-depleted aromatase-expressing cells were treated for 24 hours with the drug combinations indicated. Whole-cell extracts were assessed for expression of various proteins indicated by using immunoblotting. (A) Effect of RAD001 alone or in combination with the endocrine agents on S6 kinase, ERK1/2, and AKT for the selected cell lines. (B) Effect of RAD001 on IGF1-R signalling. (C) Effect of RAD001 on HER signaling. Figures below each panel, where shown, represent semiquantitative changes in protein expression relative to actin.Back to article page