NOTCH1 inhibition in vivo results in mammary tumor regression and reduced mammary tumorsphere-forming activity in vitro

Table 1 Limiting-dilution analysis of primary MMTV-tTA/TOP-ICN1 tumor cells

Number ofinjected cells	Number of micedeveloping tumors	Average latency(days)
10⁶	4/4	15.75 (± 3.5)
10⁵	7/7	20 (± 6.3)
10⁴	11/12	42 (± 13.6)
10³	6/14	43 (± 11.2)
10²	0/10	>100
Repopulating frequency (95% confidence interval), 1 in 2,978 (1/5,689 to 1/1,559).

Number of positive outgrowths is shown as the number of mice developing tumors per the number of mice injected. Serial dilutions of primary tumor cells were resuspended in Matrigel, transplanted into the thoracic mammary fat pads of nude mice; transplanted mice were monitored for disease. Data are pooled from four independent mammary tumors. Tumor-repopulating frequency was calculated by using L-Calc limiting-dilution analysis software.

ISSN: 1465-542X