Ets-1 mediates NO-induced MMP activity and cellular invasion. Total MMP and MMP-7 expression (a) in conditioned media from MDA-MB-468 cells transfected with control or Ets-1 siRNA and treated with DETANO. MMP expression is normalized to total cellular protein. (b) Total MMP activity in serum-starved cells transfected with control or Ets-1 siRNA and treated with DETANO. Activity is shown as fold change compared to untreated, control cells. (c) MDA-MB-231 cell invasion in response to control or Ets-1 siRNA transfection and DETANO exposure. Significance for expression, activity and invasion data were determined by t-test (*P < 0.05, **P < 0.01). DETANO, diethlylenetriamine NONOate; Ets-1, erythroblastosis virus E26 oncogene homolog 1; MMP, matrix metalloproteinase.