Kaplan-Meier analysis for Menacalc in sub-populations based on nodal status and ER status in two primary breast cancer cohorts. Each cohort was divided into quartiles and disease specific survival of the highest quartile of expression was compared to the remaining patient population. The number of patients in each group is shown followed by the number of patient deaths due to disease. Log-rank P-values are shown for 20-year follow-up. Only Menacalc scores in node positive and ER negative sub-populations are statistically significant in cohort 1 (P = 0.0039 and 0.0004 respectively).