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Figure 1 | Breast Cancer Research

Figure 1

From: Reversal by RARα agonist Am580 of c-Myc-induced imbalance in RARα/RARγ expression during MMTV-Myc tumorigenesis

Figure 1

Retinoic acid receptor (RAR)γ cooperates in Myc-induced tumorigenesis and down-regulation of RARβ2 and CRBP1. A) Quantitative (Q)-PCR analysis of RARγ1, γ2 and β2 expression in normal (FVB mammary epithelial cells, MEC) and hyperplastic (Myc MEC) mammary epithelium. B) Western blot analysis of the RAR isotypes in FVB mammary gland epithelium and MMTV-Myc tumors (three primary cultures/experimental group). C) Western blot analysis of RARγ and CRBP1 in Myc tumors and in normal FVB mammary epithelium. D) Q-PCR analysis of RARβ2, RARγ1, RARγ2 and CRBP1 expression in FVB cells transfected with a vector control or c-Myc. E) Induction of CRBP1 protein by Am580. Primary cultured Myc MECs or those stably transfected with shRARγ (Figure S1 in Additional file 1) were treated with 200 nM Am580 for 48 h. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein was used as a loading control.

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