Figure 7From: A single proteolytic cleavage within the lower hinge of trastuzumab reduces immune effector function and in vivo efficacyDetection of single hinge cleaved trastuzumab and matrix metalloproteinases in breast cancer patient samples. (A) Same amounts of enriched IgGs from each sample were separated by SDS-PAGE under nonreducing/denaturing conditions. Arrow, single hinge cleaved trastuzumab (scIgG-T) as referenced to standard preparation in the right lane. (B) Detection of matrix metalloproteinase (MMP) expression in breast cancer patient tumor tissues and normal adjacent 2 cm tissue using a reverse protein array method (n = 4). *P < 0.05 and **P < 0.005. MW, molecular weight.Back to article page