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Figure 4 | Breast Cancer Research

Figure 4

From: Identification of novel human receptor activator of nuclear factor-kB isoforms generated through alternative splicing: implications in breast cancer cell survival and migration

Figure 4

Receptor activator of NF-kB (RANK)-c tumor necrosis factor receptor superfamily ( TNFRSF-11A_Δ7,8,9 ) isoform has a dominant negative effect on RANK/RANK ligand (RANKL)-dependent NF-kB activation. A. Expression vectors containing wild type (wt) RANK or its isoforms were transfected in combination with a NF-kB-responsive luciferase reporter construct into 293T cells. Luciferase activity in mock-transfected cells was set to 1. RNA was extracted from transfected cells and mRNA expression levels of the indicated isoforms were quantified by PCR (right panel) B. Expression vectors containing RANK and RANK-c were co-transfected (at the indicated amounts) with a NF-kB-responsive luciferase reporter construct into 293T cells. P < 0.001 between asterisk-indicated samples and wt RANK luciferase levels (untreated and RANKL-treated, respectively). RNA was again extracted from transfected cells and mRNA expression levels of the indicated isoforms were quantified by PCR (right panel).

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