Identification and mRNA expression profile of TNFRSF11A gene variants in normal tissue. A. Agarose gel electrophoresis of the PCR products using primers P1 and P2 on cDNA from human peripheral blood mononuclear cells (PBMCs) and the graphical representation of the splice products identified. B. Agarose gel electrophoresis of PCR products depicting TNFRSF11A variant distribution from a panel of human normal tissue RNAs and MDA-MB-468 as a control. C. Quantitative RT-PCR of the novel splice variants and wild type (wt) receptor activator of NF-kB (RANK) from a panel of human normal tissue RNAs. Data normalization was carried out against the GAPDH housekeeping gene.