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Figure 1 | Breast Cancer Research

Figure 1

From: Human pituitary tumor-transforming gene 1 overexpression reinforces oncogene-induced senescence through CXCR2/p21 signaling in breast cancer cells

Figure 1

hPTTG1 overexpression reinforces senescence and senescence-associated secretory phenotype (SASP). (A) Cell lysates of MDA-MB-231, MCF-7, and MCF-10A cells were collected for immunoblot analysis. (B) MCF-7 cells stably transfected with pcDNA3.1 (Mock; MCF-7Mock) or pcDNA3.1-hPTTG1 (hPTTG1; MCF-7hPTTG1) were orthotopically injected into fourth mammary fat pads of SCID mice. The primary tumor weights were measured 18 weeks after implantations. Representative tumors are shown in the upper panel. Quantitative results are shown in the lower panel (n = 6 per group). The results are presented as the mean ± SEM. *P < 0.05. (C) Tumor growth was measured weekly after orthotopic implantations of MCF-7 cells stably transfected with pcDNA3.1 (Mock) or pcDNA3.1-hPTTG1 (hPTTG1). The experiments were terminated 18 weeks after implantation. Representative tumors are shown in (B). n = 6 per group per time point. Each time point shows the mean ± SEM. *P < 0.05. (D) MCF-7 and MCF-10A cells stably transfected with pcDNA3.1 (Mock) or pcDNA3.1-hPTTG1 (hPTTG1) were plated for the senescence assay. The percentage of positively stained cells is indicated to the right of the respective images. Data represent mean ± SEM (n = 3). ***P < 0.0001. (E) Cell lysates were collected from MCF-7 and MCF-10A cells stably transfected with pcDNA3.1 (Mock) or pcDNA3.1-hPTTG1 (hPTTG1) for immunoblot analysis. WT, untransfected cells. (F) Conditioned media were collected from MCF-7 cells stably transfected with pcDNA3.1 vector (Mock) or pcDNA3.1-hPTTG1 (hPTTG1) for cytokine array analysis. The differences are indicated with boxes. (G) The pixel intensity of (F) was analyzed by using Image J software.

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