Reporter gene expression assays modulated by hsa-mir-509-3p with constructs containing 362-bp of CD44 3'UTR. Representative graph of luciferase activity of variant allele on luciferase reporter genes bearing 3' UTR segments from Human CD44 in 293T (A) and MCF-7 cells (B). Results are shown as percentage relative to luciferase activity (Renilla luciferase activity was measured and normalized to Firefly luciferase). (C) Relative luciferase activity of the psiCHECK-2-CD44-3'UTR-C-allele and psiCHECK-2-CD44-T-allele constructs co-transfected with 40 pmol hsa-mir-509-3p and inhibitor. Assay was performed in 293T and MCF-7 cells. Six replicates for each group and the experiment repeated at least three times. Data are mean ± SE. *P < 0.01 compared with C allele.