Figure 6
Epithelial-to-mesenchymal transition gene expression changes were seen between tumors differing in invasive phenotype. Gene expression changes detected on an epithelial-to-mesenchymal transition (EMT) quantitative PCR array were determined upon comparison of transforming growth factor-beta receptor II knockout (TβRII KO) isolated epithelia with transforming growth factor-beta receptor II control (TβRIIfl/fl) isolated epithelia. (A) Identification of target genes was found by fold-change values. All highlighted genes were statistically significant (P < 0.05) and conform to the criteria of either 10-fold or greater upregulation or downregulation when comparing the TβRII KO laser capture microdissection (LCM) epithelia with the TβRIIfl/fl epithelia. (B) Epithelial purity of all LCM samples was confirmed when comparing PyVmT or EpCAM epithelial marker expression with that of the FAP fibroblast marker. All expression values were compared with FAP expression in the TβRIIfl/fl LCM sample. (C) Similar amounts of epithelia, as quantified by expression of PyVmT and EpCAM epithelial markers, were found in TβRII KO and TβRIIfl/fl LCM samples. (D) Array target gene expression (identified in (A)) of TβRII KO LCM samples, as compared with that of TβRIIfl/fl LCM samples, is shown with associated statistics. fib, fibroblasts.