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Figure 4 | Breast Cancer Research

Figure 4

From: Lack of transforming growth factor-β signaling promotes collective cancer cell invasion through tumor-stromal crosstalk

Figure 4

Epithelial cell transforming growth factor-beta signaling disrupted maintenance of E-cadherin/p120/β-catenin membrane localization at adherens junctions. All images were taken of in ovo tumors (asterisks, tumor-stromal regions; arrows, single cells with protein mislocalization). (A) Immunohistochemistry showed that E-cadherin was mislocalized in tumor-stromal regions in which single cells were found in transforming growth factor-beta receptor II control (TβRIIfl/fl) tumors (top and bottom left panels). Collective clusters in the same regions exhibited E-cadherin membrane localization in transforming growth factor-beta receptor II knockout (TβRII KO) tumors (top and bottom right panels). (B), (C), (D) Immunofluorescence for p120, E-cadherin, and β-catenin revealed mislocalization of their expression in stromal areas of TβRIIfl/fl tumors but maintenance in TβRII KO tumors. Cytokeratin 8/18 was used as a marker for carcinoma cell identification. DAPI, 4',6-diamidino-2-phenylindole; fib, fibroblasts.

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