Paracrine interactions between primary human macrophages and human fibroblasts enhance murine mammary gland humanization in vivo

Table 1 Time course of real time-PCR detection of human macrophages in humanized glands

	CD14	SD	CD68	SD	GAPDH	SD
Day 2
+ E₂	No CT		No CT		22.28	0.26
+ Macrophages	23.88	0.10	28.18	0.24	21.59	0.24
+ E₂ and Macrophages	26.36	0.16	28.52	1.50	22.69	0.23
Day 6
+ E₂	No CT		No CT		22.48	0.31
+ Macrophages	28.37	3.40	30.02 ^a	1.39	20.88	0.70
+ E₂ and Macrophages	27.65	4.92	30.01^a	3.57	19.69	0.91
Day 10
+ E₂	No CT		No CT		19.16	0.33
+ Macrophages	31.05^a	0.81	30.81^a	1.55	18.51	0.15
+ E₂ and Macrophages	29.43	1.51	31.28 ^a	1.12	18.65	1.13
Controls
Human monocytes	14.4	0.14	16.3	0.16	20.59	0.07
Human macrophages	16.5	0.16	20.2	0.09	21.00	0.20
Murine mammary gland	No CT		No CT		21.45	0.14

^aCycle Threshold (CT) values over 30 were not considered positive for a detectable signal. Analysis was terminated at 32 cycles. E₂, ectopic estradiol treatment; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

ISSN: 1465-542X