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Table 1 Time course of real time-PCR detection of human macrophages in humanized glands

From: Paracrine interactions between primary human macrophages and human fibroblasts enhance murine mammary gland humanization in vivo

  CD14 SD CD68 SD GAPDH SD
Day 2       
+ E2 No CT   No CT   22.28 0.26
+ Macrophages 23.88 0.10 28.18 0.24 21.59 0.24
+ E2 and Macrophages 26.36 0.16 28.52 1.50 22.69 0.23
Day 6       
+ E2 No CT   No CT   22.48 0.31
+ Macrophages 28.37 3.40 30.02 a 1.39 20.88 0.70
+ E2 and Macrophages 27.65 4.92 30.01a 3.57 19.69 0.91
Day 10       
+ E2 No CT   No CT   19.16 0.33
+ Macrophages 31.05a 0.81 30.81a 1.55 18.51 0.15
+ E2 and Macrophages 29.43 1.51 31.28 a 1.12 18.65 1.13
Controls       
Human monocytes 14.4 0.14 16.3 0.16 20.59 0.07
Human macrophages 16.5 0.16 20.2 0.09 21.00 0.20
Murine mammary gland No CT   No CT   21.45 0.14
  1. aCycle Threshold (CT) values over 30 were not considered positive for a detectable signal. Analysis was terminated at 32 cycles. E2, ectopic estradiol treatment; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
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