Figure 5From: Paracrine interactions between primary human macrophages and human fibroblasts enhance murine mammary gland humanization in vivoEnhanced primary breast fibroblast invasion in the presence of human macrophages conditioned media. Primary human macrophages were treated +/- estrogen (10-10 M) in 2% charcoal-stripped serum-containing media and 24 hr-conditioned media were collected. Primary human breast fibroblasts were seeded in serum-free media into Matrigel invasions chambers in triplicate. Chemoattractants were 2% charcoal-stripped serum (control), control media + estrogen (10-10 M), macrophage-conditioned media, or conditioned media from macrophages treated with estrogen. (A) 24 hr post-seeding, invaded cells were stained with toluidine blue (top panels), imaged and quantified using NIH Image J software (measurement highlighted in red, bottom panels). (B) Data represent average area measurement of invaded cells +/- standard error (SE) of five independent experiments. *P < 0.01 above control or E2 treatment. Ï•, macrophage conditioned media; E2, estrogen treatment.Back to article page