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Figure 3 | Breast Cancer Research

Figure 3

From: Paracrine interactions between primary human macrophages and human fibroblasts enhance murine mammary gland humanization in vivo

Figure 3

Macrophages stimulate primary breast fibroblast proliferation. Primary human monocytes/macrophages were isolated, differentiated, treated +/- estrogen (10-10 M) for 24 hr and conditioned media were collected. Primary human breast fibroblasts were placed in 2% charcoal-stripped serum-containing media 24 hr prior to treatment +/- estrogen or with macrophage-conditioned media +/- estrogen for three days, then trypsinized and counted. Data represent four independent experiments with different fibroblasts and macrophage isolations used for each assay. *P < 0.01. ϕ, macrophage conditioned media; E2, estrogen treatment.

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