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Figure 5 | Breast Cancer Research

Figure 5

From: γ-Secretase inhibition promotes cell death, Noxa upregulation, and sensitization to BH3 mimetic ABT-737 in human breast cancer cells

Figure 5

Notch inhibition decreased mammosphere formation. (A) MCF7 and BT549 cell lines were evaluated for their mammosphere-formation capacity with GSIXII treatment with indicated concentrations. Represented data are the means of MFU% compared with the mock-treated condition ± SEM, from three independent experiments. (B) The capacity of MCF7 and BT549 cells to form mammospheres at the first (1st), second (2nd), and third (3rd) generations was assessed in the presence or not of 8 μM GSIXII added at the beginning of the MFU assay. Represented data are from three independent experiments. Statistical analysis compared mammosphere formation in each serial generation with its mock-treated control. (C) SiRNA targeting Noxa (black bar) significantly rescued mammosphere formation in BT549 and MCF7 cells on GSIXII or mock treatment compared with siRNA control (white bar).

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