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Table 1 Mass spectrometry identification of proteins differentially expressed between wild type MCF7 cells and MCF7 cells stably transfected with 17β-HSD1 (MCF7-17βHSD1).

From: 17beta-hydroxysteroid dehydrogenase type 1 modulates breast cancer protein profile and impacts cell migration

Spot FC Description UniProt number MW exp/
pred
pI
exp
Pep Function and/or biological process
Spot downregulated in MCF7-17βHSD1 as compared to WT MCF7
4183 2.7 Cathepsin D P07339 28/45 5.0 20 Proteolysis, pathogenesis of diseases (breast cancer)
   Ezrin-radixin-moesin-binding phosphoprotein 50 O14745 58/39 5.3 17 Wnt signaling pathway
3252 2.3 Neudesin Q9UMX5 19/19 4.7 2 Neuronal differentiation and proliferation
1703 2.2 Ribonuclease/angiogenin inhibitor 1 (RNH1)(3177)a P13489 58/50 4.6 24 Regulation of angiogenesis, mRNA catabolism
   BRCA2 and CDKN1A interacting protein
(BCCIP)a
Q9P287 58/36 4.6 4 Promote cell cycle arrest
   Cell division cycle protein 123 homolog O75794 58/39 4.6 2 Required for S phase entry of the cell cycle
Spot unique to WT MCF7
2617 U Poly(rC)-binding protein 2 Q15366 32/39 6.4 11 RNA binding
   Purine nucleoside phosphorylase P00491 32/32 6.4 6 DNA modification
   BTB/POZ domain-containing protein KCTD15 Q96SI1 32/32 6.4 4 Potassium ion transport
   RING finger protein 114 Q9Y508 32/26 6.4 2 Cell differentiation
4335 U Peptidyl-prolyl cis-trans isomerase E Q9UNP9 35/33 5.4 10 Protein folding, mRNA splicing
   Transgelin-2 P37802 20/22 5.6 8 Muscle development
   Splicing factor, arginine/serine-rich 2 Q01130 35/25 5.4 2 mRNA processing
3039 U RAB11B protein A5YM50 24/25 5.7 11 Protein transport, signal transduction
   Peroxiredoxin-2a P32119 24/22 5.7 6 Cell redox regulation, anti-
apoptosis
   Splicing factor, arginine/serine-rich 3 P84103 24/19 5.7 2 RNA processing in relation with cell proliferation
Spot upregulated in MCF7-17ΒHSD1 as compared to WT MCF7
2714 2.5 Exosome complex exonuclease RRP41 Q9NPD3 29/26 6.2 3 rRNA processing
   Enoyl-CoA hydratase, mitochondrial P30084 29/31 6.2 6 Fatty acid and lipid metabolism
   Heat shock 70 kDa protein 1 P08107 29/70 6.2 5 Stress response
   Eukaryotic translation initiation factor 4H Q15056 29/27 6.2 4 Host-virus interaction, protein biosynthesis
2496 4.9 Proliferating cell nuclear antigen (PCNA)a P12004 32/29 4.6 16 DNA replication
5474 4.0 Myosin regulatory light chain 2, nonsarcomeric P19105 20/20 4.7 5 Cytokinesis, receptor capping, cell locomotion
2300 3.3 60S acidic ribosomal protein P0(2305) A8K4Z4 40/34 5.6 6 Ribosome biogenesis, translation elongation
Spot unique to MCF7-17ΒHSD1
2305 U 17β-hydroxysteroid dehydrogenase type 1(2300) P14061 41/35 5.4 22 Steroid biosynthesis
3177 U Metastasis inhibition factor nm23 (nm23-H1)a P15531 21/17 5.9 6 Cell cycle and proliferation,
nucleotide metabolism
4667 U 60S ribosomal protein L11 P62913 21/20 5.9 2 Binds to 5S ribosomal RNA
4678 U S-phase kinase-associated protein 1 (SKP1)a P63208 20/19 4.6 2 Ubl conjugation pathway
  1. The function and/or biological process were obtained from the UniProt database [19].
  2. Spot, spot number; FC, fold change; U, unique; MW, molecular weight (kDa); pI exp, isoelectric point as determined from the two-dimensional gel experiments; Pep, number of unique peptides. aProteins used for RT-qPCR validation. The number after the protein name indicates the additional spot in which the protein was found.