Table 1 Mass spectrometry identification of proteins differentially expressed between wild type MCF7 cells and MCF7 cells stably transfected with 17β-HSD1 (MCF7-17βHSD1).

Spot downregulated in MCF7-17βHSD1 as compared to WT MCF7

4183

2.7

Cathepsin D

P07339

28/45

5.0

20

Proteolysis, pathogenesis of diseases (breast cancer)

Ezrin-radixin-moesin-binding phosphoprotein 50

O14745

58/39

5.3

17

Wnt signaling pathway

3252

2.3

Neudesin

Q9UMX5

19/19

4.7

2

Neuronal differentiation and proliferation

1703

2.2

Ribonuclease/angiogenin inhibitor 1 (RNH1)₍₃₁₇₇₎^a

P13489

58/50

4.6

24

Regulation of angiogenesis, mRNA catabolism

BRCA2 and CDKN1A interacting protein

(BCCIP)^a

Q9P287

58/36

4.6

4

Promote cell cycle arrest

Cell division cycle protein 123 homolog

O75794

58/39

4.6

2

Required for S phase entry of the cell cycle

Spot unique to WT MCF7

2617

U

Poly(rC)-binding protein 2

Q15366

32/39

6.4

11

RNA binding

Purine nucleoside phosphorylase

P00491

32/32

6.4

6

DNA modification

BTB/POZ domain-containing protein KCTD15

Q96SI1

32/32

6.4

4

Potassium ion transport

RING finger protein 114

Q9Y508

32/26

6.4

2

Cell differentiation

4335

U

Peptidyl-prolyl cis-trans isomerase E

Q9UNP9

35/33

5.4

10

Protein folding, mRNA splicing

Transgelin-2

P37802

20/22

5.6

8

Muscle development

Splicing factor, arginine/serine-rich 2

Q01130

35/25

5.4

2

mRNA processing

3039

U

RAB11B protein

A5YM50

24/25

5.7

11

Protein transport, signal transduction

Peroxiredoxin-2^a

P32119

24/22

5.7

6

Cell redox regulation, anti-

apoptosis

Splicing factor, arginine/serine-rich 3

P84103

24/19

5.7

2

RNA processing in relation with cell proliferation

Spot upregulated in MCF7-17ΒHSD1 as compared to WT MCF7

2714

2.5

Exosome complex exonuclease RRP41

Q9NPD3

29/26

6.2

3

rRNA processing

Enoyl-CoA hydratase, mitochondrial

P30084

29/31

6.2

6

Fatty acid and lipid metabolism

Heat shock 70 kDa protein 1

P08107

29/70

6.2

5

Stress response

Eukaryotic translation initiation factor 4H

Q15056

29/27

6.2

4

Host-virus interaction, protein biosynthesis

2496

4.9

Proliferating cell nuclear antigen (PCNA)^a

P12004

32/29

4.6

16

DNA replication

5474

4.0

Myosin regulatory light chain 2, nonsarcomeric

P19105

20/20

4.7

5

Cytokinesis, receptor capping, cell locomotion

2300

3.3

60S acidic ribosomal protein P0₍₂₃₀₅₎

A8K4Z4

40/34

5.6

6

Ribosome biogenesis, translation elongation

Spot unique to MCF7-17ΒHSD1

2305

U

17β-hydroxysteroid dehydrogenase type 1₍₂₃₀₀₎

P14061

41/35

5.4

22

Steroid biosynthesis

3177

U

Metastasis inhibition factor nm23 (nm23-H1)^a

P15531

21/17

5.9

6

Cell cycle and proliferation,

nucleotide metabolism

4667

U

60S ribosomal protein L11

P62913

21/20

5.9

2

Binds to 5S ribosomal RNA

4678

U

S-phase kinase-associated protein 1 (SKP1)^a

P63208

20/19

4.6

2

Ubl conjugation pathway