Laminin-332 upregulation and integrin β4 neoexpression suppressed caspase-3 activity in fibroblasts. (A) Caspase-3 activity of fibroblasts under anoikis conditions in the absence or presence of integrin β4 and/or stimulation with CM from cancer cells. Black bar, CAF; gray bar, InF; white bar, NBF; gray box, integrin β4-expressing fibroblasts. (B) Caspase-3 activity according to inhibition of integrin β1 (dark-gray bar), β4 (light-gray bar), or laminin-332 (white bar). The black bar shows the isotype control. (C) Effect of laminim-322 treatment on caspase-3 activity in CAFs. Black bar, untreated CAFs; white bar, laminin-322-treated CAFs. Results are expressed as mean ± SD. *P < 0.05; **P < 0.03 versus RSM in poly-HEMA-coated wells. Results are averages of three separate experiments.