Growth curves of control and knockdown cells. Mean ± SEM for (A) MCF7 and (B) T47D control and plakoglobin knockdown cells. (C, D) Relative expression of plakoglobin over the 96-hour time course of the cell-proliferation experiment in control and knockdown MCF7 and T47D cells, respectively. (E, F) The effects of plakoglobin expression on tumor cell adhesion. (E) Spheroid formation and (F) the percentage of cells adhering to a confluent monolayer of cells of an identical cell type after 2 hours of co-culture. Data shown are expressed as mean ± SEM (*P < 0.005, by one-way ANOVA followed by the Dunnett two-sided multiple comparison test).