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Figure 3 | Breast Cancer Research

Figure 3

From: Bone marrow-derived, alternatively activated macrophages enhance solid tumor growth and lung metastasis of mammary carcinoma cells in a Balb/C mouse orthotopic model

Figure 3

M2-polarized macrophages enhance leukocyte infiltration into tumor tissues and monocyte migration in vitro. (A) 4T1 cells and/or M2-polarized macrophages (M2-Mϕs) were injected into the mammary fat pad of female Balb/C mice. M2-Mϕs were labeled with 3,3'-dioctadecyloxacarbocyanine perchlorate/DiOC18 (DiO) (green) prior to the injection. Cryostat sections of tumor were stained with anti-CD45 antibody (red), and nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue). (B) 4T1 cells and/or M2-Mϕs were plated in 12-well plates, and 24-hour-conditioned media (CM) were collected for trans-well migration assays. The migration of bone marrow-derived monocytes was estimated using the CM as a chemoattractant. The migrated monocytes were quantified by counting the DAPI-stained cells. Each bar represents the mean ± standard error of the mean of three independent experiments performed in duplicate. Means without the same lowercase letter differ, P < 0.05.

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Breast Cancer Research

ISSN: 1465-542X

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